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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
09/04/2015 |
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Data da última atualização: |
09/04/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ERBANO, M.; SCHUHLI, G. S. e; SANTOS, É. P. dos. |
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Afiliação: |
Marianna Erbano, Pós-graduanda da UFPR; GUILHERME SCHNELL E SCHUHLI, CNPF; Élide Pereira dos Santos, UFPR. |
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Título: |
Genetic variability and population structure of Salvia lachnostachys: implications for breeding and conservation programs. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
International Journal of Molecular Sciences, v. 16, p. 7839-7850, 2015. |
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DOI: |
10.3390/ijms16047839 |
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Idioma: |
Inglês |
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Conteúdo: |
The genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the first molecular assessment for this endemic species with medicinal proprieties and the results can guide for subsequent bioprospection, breeding programs or conservation actions. MenosThe genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the fir... Mostrar Tudo |
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Palavras-Chave: |
Estrutura populacional; Intraspecific diversity; ISSR; Labiatae; Melhoramento genético; Salvia lachnostachys; Variabilidade genética. |
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Thesagro: |
Conservação. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122031/1/2015-API-GuilhermeS-GeneticVariability.pdf
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Marc: |
LEADER 02492naa a2200253 a 4500
001 2013073
005 2015-04-09
008 2015 bl uuuu u00u1 u #d
024 7 $a10.3390/ijms16047839$2DOI
100 1 $aERBANO, M.
245 $aGenetic variability and population structure of Salvia lachnostachys$bimplications for breeding and conservation programs.$h[electronic resource]
260 $c2015
520 $aThe genetic diversity and population structure of Salvia lachnostachys Benth were assessed. Inter Simple Sequence Repeat (ISSR) molecular markers were used to investigate the restricted distribution of S. lachnostachys in Parana State, Brazil. Leaves of 73 individuals representing three populations were collected. DNA was extracted and submitted to PCR-ISSR amplification with nine tested primers. Genetic diversity parameters were evaluated. Our analysis indicated 95.6% polymorphic loci (stress value 0.02) with a 0.79 average Simpson?s index. The Nei-Li distance dendrogram and principal component analysis largely recovered the geographical origin of each sample. Four major clusters were recognized representing each collected population. Nei?s gene diversity and Shannon?s information index were 0.25 and 0.40 respectively. As is typical for outcrossing herbs, the majority of genetic variation occurred at the population level (81.76%). A high gene flow (Nm = 2.48) was observed with a correspondingly low fixation index. These values were generally similar to previous studies on congeneric species. The results of principal coordinate analysis (PCA) and of arithmetic average (UPGMA) were consistent and all three populations appear distinct as in STRUCTURE analysis. In addition, this analysis indicated a majority intrapopulation genetic variation. Despite the human pressure on natural populations our study found high levels of genetic diversity for S. lachnostachys. This was the first molecular assessment for this endemic species with medicinal proprieties and the results can guide for subsequent bioprospection, breeding programs or conservation actions.
650 $aConservação
653 $aEstrutura populacional
653 $aIntraspecific diversity
653 $aISSR
653 $aLabiatae
653 $aMelhoramento genético
653 $aSalvia lachnostachys
653 $aVariabilidade genética
700 1 $aSCHUHLI, G. S. e
700 1 $aSANTOS, É. P. dos
773 $tInternational Journal of Molecular Sciences$gv. 16, p. 7839-7850, 2015.
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Registro original: |
Embrapa Florestas (CNPF) |
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| Registros recuperados : 56 | |
| 3. |  | SABADIN, G.; SCHUHLI, G. S. e. Prospecção de variedades de guabiroba por meio de marcadores ISSR. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 12., 2013, Colombo. Anais. Colombo: Embrapa Florestas, 2013. (Embrapa Florestas. Documentos, 253). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 5. | | WOLFF, L. F.; SCHUHLI, G. S. e. Eucaliptos e abelhas. In: OLIVEIRA, E. B. de; PINTO JUNIOR, J. E. (Ed.). O eucalipto e a Embrapa: quatro décadas de pesquisa e desenvolvimento. Brasília, DF: Embrapa, 2021. cap. 16. p. 687-699.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Clima Temperado; Embrapa Florestas. |
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| 13. | | BUSCH, E. F.; BROWN, G. G.; SCHUHLI, G. S. e. Comparação entre os métodos "salting out" e CTAB na extração de DNA de minhocas. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 12., 2013, Colombo. Anais. Colombo: Embrapa Florestas, 2013. (Embrapa Florestas. Documentos, 253). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos., 2013.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 17. | | SILVEIRA, S. S.; QUOIRIN, M. G. G.; SCHUHLI, G. S. e. Análise de potencial de regeneração in vitro em plantas de guanandi por marcadores moleculares dominantes. In: EVENTO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA FLORESTAS, 12., 2013, Colombo. Anais. Colombo: Embrapa Florestas, 2013. (Embrapa Florestas. Documentos, 253). Editores técnicos: Marcílio José Thomazini, Elenice Fritzsons, Patrícia Raquel Silva, Guilherme Schnell e Schuhli, Denise Jeton Cardoso, Luziane Franciscon. EVINCI. Resumos.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Florestas. |
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| 18. | | SCHUHLI, G. S. e; PENTEADO, S. C.; BARBOSA, L. R.; REIS FILHO, W.; IEDE, E. T. A review of the introduced forest pests in Brazil. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 5, p. 397-406, maio 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Florestas; Embrapa Unidades Centrais. |
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| Registros recuperados : 56 | |
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